|
KMID : 0616120170480030179
|
|
Korean Journal of Pharmacognosy
2017 Volume.48 No. 3 p.179 ~ p.186
|
|
|
Protective Effects of Pyrrosiae Folium on the 2% Glucose-Induced Toxicity in Caenorhabditis elegans
|
|
Kim Bong-Seok
Lee Byung-Ju
Lee Hyun-Joo
An Soon-Young
Park Zi-Won
Yoon Seon-Hwa
Oh Mi-Jin
Kwon Jin
Lee Se-Yeoun
Cha Dong-Seok
Oh Chan-Ho
Jeon Hoon
|
|
|
Abstract
|
|
|
|
Pyrrosia lingua which belongs to Polypodiaceae has been used as a traditional medicine for the treatment of urinary system inflammation, urination disorder, and bronchitis. However, there are not enough phytochemical and pharmacological studies of P. lingua up to now. Here in this study, the protective effect of MeOH extract of whole plant of Pyrrosia lingua (MPL) against 2% glucose-induced toxicity was investigated using Caenorhabditis elegans (C. elegans) model system. We found that MPL significantly extended the lifespan of wild-type nematode under normal culture condition. MPL also effectively recovered the decreased lifespan caused by 2% glucose-toxicity. In addition, MPL efficiently attenuated the increased glucose concentration inside of nematode. Further studies evaluating diabetes-related factors revealed that MPL reduced both intracellular ROS and lipid accumulation which were up-regulated under 2% glucose supplement condition. Our data also showed that MPL improved the 2% glucose-induced shortened body movement of nematode. Lastly, we carried out genetic studies using several single gene knockout mutants to establish the possible target of MPL. Our results demonstrated that genes such as daf-2 and daf-16 were responsible for the protective activity of MPL against 2% glucose-induced toxicity. These results indicate that MPL exerts protective action against 2% glucose via regulation of insulin/IGF-1 sinaling pathway and FOXO activation.
|
|
|
KEYWORD
|
|
|
Pyrrosia lingua , Anti-diabetes , Glucose Toxicity , Lifespan
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|